Reference assembly refinement reveals additional variants at the chromosome 5 milk fat percentage locusWe noted that the cluster of 17 highly associated MGST1 variants were adjacent to a reference sequence gap present in both UMD3.1 and Btau_4.6.1 assemblies. To identify potentially functional variants located within the 364bp gap (UMD3.1), we used a sequential re mapping, gap extension, and de novo assembly strategy to reveal an additional 17 variants in the 556 reference animals (Materials and Methods). P=3.61066; Fig.
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